State and Local Brownfields Revitalization Act of 2000 - Requires the Secretary of the Army to implement a program to provide assistance to State, regional, and local governments in the remediation and restoration of brownfield sites in cases in which the remediation and restoration would contribute to the quality, conservation, and sustainable use of U.S. waterways and watershed ecosystems. Defines a "brownfield site" as a parcel of real property, the expansion, redevelopment, or reuse of which is complicated by the presence or potential presence of a hazardous substance.

Permits assistance only for project sites that are publicly-owned. Limits the Federal share of project costs to 65 percent. Sets forth provisions regarding the non-Federal share of project costs. Requires the non-Federal interest to pay 100 percent of operation and maintenance costs of a remediated site. Limits the total amount of assistance for any one project.

Authorizes appropriations.