Florida Keys Water Quality Improvements Act of 2000 - Amends the Federal Water Pollution Control Act to authorize the Administrator of the Environmental Protection Agency to make grants to the Florida Keys Aqueduct Authority, agencies of municipalities of Monroe County, Florida, and other public agencies of the State of Florida or Monroe County for the planning and construction of treatment works to improve water quality in the Florida Keys National Marine Sanctuary. Sets forth project requirements. Limits the Federal share of project costs to 75 percent.

Expresses the sense of Congress, in the case of equipment or products authorized to be purchased with financial assistance provided under this Act, that agencies receiving such assistance should, in expending such assistance, purchase only equipment and products produced in the United States. Requires such agencies to report any expenditures on foreign-produced items to Congress.

Authorizes appropriations.