Alien Smuggling Prevention and Enforcement Act of 1999 - Directs the Attorney General to make specified increases in the number of full-time, active duty Immigration and Naturalization Service personnel assigned to combat alien smuggling.

Directs the United States Sentencing Commission to: (1) provide in its sentencing guidelines for increased criminal sentences and fines for alien smuggling and related activities; and (2) amend its sentencing guidelines to provide that plea bargaining and other prosecutorial policy differences among districts shall not be a ground for sentence imposition outside the applicable guidelines.

Authorizes additional appropriations for such alien smuggling enforcement activities.